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ORIGINAL ARTICLE
Year : 2022  |  Volume : 34  |  Issue : 1  |  Page : 50-55

Repeatability of corneal densitometry measurements using a scheimpflug camera in healthy normal corneas


1 Noor Ophthalmology Research Center, Noor Eye Hospital; Translational Ophthalmology Research Center, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Basic Sciences, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5 Noor Research Center for Ophthalmic Epidemiology, Noor Eye Hospital, Tehran, Iran
6 Translational Ophthalmology Research Center, Tehran University of Medical Sciences, Tehran, Iran
7 Translational Ophthalmology Research Center, Tehran University of Medical Sciences; Noor Ophthalmology Research Center, Noor Eye Hospital, Tehran, Iran

Correspondence Address:
Hassan Hashemi
Translational Ophthalmology Research Center, Tehran University of Medical Sciences, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/joco.joco_173_21

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Purpose: To determine the repeatability of corneal densitometry measured by the Scheimpflug imaging system. Methods: This cross-sectional study was conducted on photorefractive keratectomy candidates. One eye of each participant underwent imaging using Pentacam HR three times, 10 min apart. The repeatability of densitometry measurements was evaluated in four concentric annuli around the corneal apex and in different corneal depths. The repeatability of the measurements was evaluated using the intraclass correlation coefficient (ICC), repeatability coefficient (RC), and coefficient of variation (CV). The difference of repeatability between layers and zones was tested by tolerance index (TI). Results: Sixty eyes of sixty patients with a mean age of 27.76 ± 3.93 years were studied. Half of the participants were female (n = 30, 50%). ICC was above 0.9 in all corneal parts. The posterior layer and central zones showed the least variability of densitometry measurements considering the CV values. The RC was 2.06, 1.17, and 0.92 in anterior, central, and posterior layers, respectively. The RC was 0.88, 0.71, 1.51, and 4.56 in 0–2, 2–6, 6–10, and 10–12 mm circles, respectively. Only the reliability of densitometry in 10–12 mm annulus was statistically lower than the central zone (TI = 0.71). Conclusions: Corneal densitometry measurements provided by the Pentacam had good repeatability. The repeatability of densitometry measurements decreased from the center to the periphery (with an exception for 0–2 mm and 2–6 mm) and from the posterior to the anterior of the cornea. The reliability of the 10–12 mm zone was markedly less than other zones.


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